• Product nameAnti-PCNA antibody
    See all PCNA primary antibodies
  • Description
    Rabbit polyclonal to PCNA
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Alligator, Orangutan
  • Immunogen

    This information is considered to be commercially sensitive.

  • Positive control
    • A431 whole cell lysate.



Our Abpromise guarantee covers the use of ab19166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 19945538
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.03µg for 106 cells.

0.03 μg per 1 million cells in a 150 mcl reaction.

ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).


  • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similaritiesBelongs to the PCNA family.
  • Post-translational
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Anti-PCNA antibody images

  • Immunohistochemistry (Frozen sections) analysis of 8 μm sections of mouse prostate triple labelled with rabbit polyclonal antibody for PCNA (ab19166) (1/200, green), mouse monoclonal antibody for smooth muscle actin (red) and the nuclear stain DAPI (blue). Arrows indicate positive staining for PCNA and DAPI (i.e. nuclei of proliferating cells). L, lumen; E, epithelium; S, smooth muscle stroma. Calibration bar=200 μm.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling PCNA with ab19166 at 1/10000 (0.1µg/ml). Detection: DAB.
  • Lanes 1 - 2 : Anti-PCNA antibody (ab19166) at 1 µg/ml
    Lanes 3 - 4 : Anti-PCNA antibody (ab19166) at 0.1 µg/ml

    Lane 1 : A431 whole cell lysate at 15 µg
    Lane 2 : A431 whole cell lysate at 50 µg
    Lane 3 : A431 whole cell lysate at 15 µg
    Lane 4 : A431 whole cell lysate at 50 µg

    Predicted band size : 31 kDa

    Exposure time : 2 minutes

    ab19166 (Human PCNA antibody) in western blot in A431 whole cell lysate:

    Lane 1: 15µg lysate; 1ug/ml ab19166
    Lane 2: 50µg lysate; 1ug/ml ab19166
    Lane 3: 15µg lysate; 0.1ug/ml ab19166
    Lane 4: 50µg lysate; 0.1ug/ml ab19166

    WB exposure: 2 minutes

References for Anti-PCNA antibody (ab19166)

This product has been referenced in:
  • Chang BS  et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015). WB ; Mouse . Read more (PubMed: 25740096) »
  • Xie JH  et al. Troubleshooting immunohistochemical labelling of proliferating cell nuclear antigen (PCNA) in cryocut tissue sections of mouse prostate. J Pharmacol Toxicol Methods 61:98-101 (2010). IHC-Fr ; Mouse . Read more (PubMed: 19945538) »

See all 2 Publications for this product

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