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Synthetic peptide: DMGHLKYYLAPKIEDEEGS, corresponding to C terminal amino acids 243-261 of Human PCNA.
Our Abpromise guarantee covers the use of ab2426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/500. See Abreviews.|
|IP||Use at an assay dependent concentration.|
|WB||1/200. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with PCNA peptide (ab2427).|
|IHC-P||1/2000 - 1/3000. See Abreview.|
ab2426 staining PCNA in Mouse 14.5 dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-T and blocked with 1% BSA + 1% FBS in TBS for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris buffer, pH 9. Samples were incubated with primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab2426 staining PCNA in murine skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TRIS EDTA pH 8.2. Samples were then incubated with the primary antibody at a 1/2000 dilution for 1 hour at 37°C. An undiluted HRP-conjugated rabbit polyclonal was used as secondary antibody.
ab2426 was used for immunohistochemistry, at a 1:500 dilution in hamster neural tissue, identified with a polyclonal secondary and DAB detection kit. Review by Kevin Bath submitted 18 June 2004
ab2426 at 1/200 dilution staining rat skeletal muscle satellite stem cells by ICC/IF.
Cultured rat skeletal muscle satellite stem cells (SKMB) were 2% paraformaldehyde fixed for 15 minutes and then permealized with Triton-X100 prior to incubation with ab2426 overnight at 4°C. An Alexa-Fluor ® 488 conjugated donkey anti-rabbit (ab150073) antibody was used as the secondary.The image shows DAPI (blue-nuclear stain, upper left panel), PCNA (Green, upper right panel, showing nuclear localization in actively dividing cells), same SKMB cells -DIC (phase) image (lower left panel) and superimpose fluorescence image (lower right panel).
ab2426 staining PCNA in murine epithelial tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Wound tissue sections (0.5 µm) were cut using a microtome and collected on slides. Sections were then de-waxed in xylene and rehydrated by successive immersion in descending concentrations of alcohol. The sections were then subjected for immunofluorescence staining. Briefly, tissue sections were blocked by incubated with 5% donkey serum (ab7475) for 1 hour and washed with phosphate-buffered saline (PBS). Sections were then incubated with ab2426 at a 1/500 for 1 hour at room temperature under humidified conditions. After primary antibody incubation, sections were washed with PBS and incubated with appropriate fluorescent secondary antibodies for 1 hour at room temperature. Sections were then washed with PBS, mounted with mounting medium containing DAPI.
Image courtesy of an anonymous Abreview.
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