Overview

  • Product nameAnti-PCNA antibodySee all PCNA primary antibodies ...
  • Description
    Rabbit polyclonal to PCNA
  • SpecificityPCNA (C19) DB051 reacts with PCNA of mouse, rat, and human origin
  • Tested applicationsIHC-Fr, IP, WB, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human, Zebrafish, Marmoset (common), Apteronotus leptorhynchus
  • Immunogen

    Synthetic peptide: DMGHLKYYLAPKIEDEEGS, corresponding to C terminal amino acids 243-261 of Human PCNA.

  • Positive control
    • A431 and NIH/3T3 lysate. HeLa- nuclear lysate. Mouse heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr 1/500. See Abreviews.
IP Use at an assay dependent concentration.
WB 1/200. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with PCNA peptide (ab2427).
IHC-P 1/2000 - 1/3000. See Abreview.
ICC/IF 1/200.

See Abreview.

Target

  • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similaritiesBelongs to the PCNA family.
  • Post-translational
    modifications
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Target information above from: UniProt accession P12004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating Cell Nuclear Antigen antibody
    see all

Anti-PCNA antibody images

  • ab2426 staining PCNA in Mouse 14.5 dpc tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with TBS-T and blocked with 1% BSA + 1% FBS in TBS for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris buffer, pH 9. Samples were incubated with primary antibody (1/1000 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab2426 staining PCNA in murine skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using TRIS EDTA pH 8.2. Samples were then incubated with the primary antibody at a 1/2000 dilution for 1 hour at 37°C. An undiluted HRP-conjugated rabbit polyclonal was used as secondary antibody.

    See Abreview

  • ab2426 was used for immunohistochemistry, at a 1:500 dilution in hamster neural tissue, identified with a polyclonal secondary and DAB detection kit. Review by Kevin Bath submitted 18 June 2004

  • ab2426 at 1/200 dilution staining rat skeletal muscle satellite stem cells by ICC/IF.

    Cultured rat skeletal muscle satellite stem cells (SKMB) were 2% paraformaldehyde fixed for 15 minutes and then permealized with Triton-X100 prior to incubation with ab2426 overnight at 4°C. An Alexa-Fluor ® 488 conjugated donkey anti-rabbit (ab150073) antibody was used as the secondary.The image shows DAPI (blue-nuclear stain, upper left panel), PCNA (Green, upper right panel, showing nuclear localization in actively dividing cells), same SKMB cells -DIC (phase) image (lower left panel) and superimpose fluorescence image (lower right panel).

    See Abreview

  • ab2426 staining PCNA in murine epithelial tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Wound tissue sections (0.5 µm) were cut using a microtome and collected on slides. Sections were then de-waxed in xylene and rehydrated by successive immersion in descending concentrations of alcohol. The sections were then subjected for immunofluorescence staining. Briefly, tissue sections were blocked by incubated with 5% donkey serum (ab7475) for 1 hour and washed with phosphate-buffered saline (PBS). Sections were then incubated with ab2426 at a 1/500 for 1 hour at room temperature under humidified conditions. After primary antibody incubation, sections were washed with PBS and incubated with appropriate fluorescent secondary antibodies for 1 hour at room temperature. Sections were then washed with PBS, mounted with mounting medium containing DAPI.

  • All lanes : Anti-PCNA antibody (ab2426) at 1/200 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • All lanes : Anti-PCNA antibody (ab2426) at 1/300 dilution

    Lane 1 : Whole tissue lysate prepared from mouse heart
    Lane 2 : Whole tissue lysate prepared from mouse heart
    Lane 3 : Whole tissue lysate prepared from mouse heart
    Lane 4 : Whole tissue lysate prepared from mouse heart

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated pig anti-rabbit polyclonal at 1/3000 dilution
    developed using the ECL technique

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-PCNA antibody (ab2426)

This product has been referenced in:
  • Jia C  et al. An IP3R3- and NPY-expressing microvillous cell mediates tissue homeostasis and regeneration in the mouse olfactory epithelium. PLoS One 8:e58668 (2013). IHC ; Mouse . Read more (PubMed: 23516531) »
  • Zuniga KE  et al. The interactions of dietary tomato powder and soy germ on prostate carcinogenesis in the TRAMP model. Cancer Prev Res (Phila) 6:548-57 (2013). Mouse . Read more (PubMed: 23592738) »

See all 35 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Mouse Cell (NIH 3T3)
Specification NIH 3T3
Permeabilization Yes - PBS Tw-20 0,5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 26 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 1%BSA / 1% FBS in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris buffer pH 9
Sample Mouse Tissue sections (Mouse 14.5 dpc)
Specification Mouse 14.5 dpc
Permeabilization Yes - TBS-T
Fixative Formaldehyde
Username

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Verified customer

Submitted Sep 24 2013

Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Suypriya Sen

Verified customer

Submitted May 10 2013

Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Loading amount 50 µg
Specification heart
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 28 2012

Application Western blot
Sample Rat Tissue lysate - whole (heart)
Loading amount 50 µg
Specification heart
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 28 2012

Thank you very much for your email.

One vial of ab2712 has been added to your order, and you can pay for this order with the PO that you provided or with a credit card if you prefer. If you'd like to add a credit card to your order, please ca...

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Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2012

According to our records, ab2426 was proving difficult to use in IHC-P and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to resolve this case.
I am ...

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Thank you for your reply.


Based on when you received this product, the antibody has already exceeded our 6 month Abpromise guarantee period and I am not able to send a replacement free of charge. I have included the terms and conditions...

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Thank you for contacting Abcam regarding ab2426.


I am sorry that you have been experiencing difficulties with this antibody in ICC/IF. I have reviewed the protocol information and imageyou provided and I agree that this product is not p...

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