Overview

  • Product nameAnti-PCNA antibody
    See all PCNA primary antibodies
  • Description
    Rabbit polyclonal to PCNA
  • Specificityab53048 detects endogenous levels of total PCNA protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human PCNA. Immunogen range is from aa 61 to 110

  • Positive control
    • Human breast carcinoma tissue and HepG2 cell extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similaritiesBelongs to the PCNA family.
  • Post-translational
    modifications
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Anti-PCNA antibody images

  • All lanes : Anti-PCNA antibody (ab53048) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with immunising peptide


    Predicted band size : 29 kDa
  • ab53048 at 1/50 dilution staining PCNA in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue (in the absence and presence of the immunising peptide).

References for Anti-PCNA antibody (ab53048)

This product has been referenced in:
  • Fleming JM  et al. Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo. Breast Cancer Res 14:R97 (2012). IHC-P ; Human . Read more (PubMed: 22731827) »

See 1 Publication for this product

Product Wall

I am sorry if there was mis-communication. The customer, according to your previous e-mail, asked for a quote for PCNA antibodies which are known to be reactive with zebrafish. The two I mentioned in my previous e-mail are: ab29 - 100µg for $35...

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I am sorry to read that your customer is still having difficulty with ab53048. If I understand you correctly, the customer wants to know which antibodies we recommend for detecting PCNA in zebrafish samples. We have two: ab29 - 100µg for...

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Thank you for the protocol details and the image. I assume the customer is expecting to see more PCNA expression in a subset of cells. It is difficult, with this image, for me to identify specific regions of the embryo but I think there will be...

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Thank you for bringing this to our attention. The antibodies we offer are generally stable at 4ºC for several weeks, if not months. The recommendation for storage at -20ºC refers to long-term storage (beyond a few weeks), so it is unlikely that the del...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"