Anti-PCNA antibody (Biotin) (ab113270)


  • Product nameAnti-PCNA antibody (Biotin)
    See all PCNA primary antibodies
  • Description
    Rabbit polyclonal to PCNA (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Alligator, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 75 and 125 of Human PCNA (UniProt P12004).

  • Positive control
    • HeLa whole cell lysate


Associated products


Our Abpromise guarantee covers the use of ab113270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.03µg for 106 cells. in 150 µl total volume.

ab200208-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IP Use at 2-5 µg/mg of lysate.


  • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
  • Sequence similaritiesBelongs to the PCNA family.
  • Post-translational
    Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
  • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATLD2 antibody
    • cb16 antibody
    • Cyclin antibody
    • DNA polymerase delta auxiliary protein antibody
    • etID36690.10 antibody
    • fa28e03 antibody
    • fb36g03 antibody
    • HGCN8729 antibody
    • MGC8367 antibody
    • Mutagen-sensitive 209 protein antibody
    • OTTHUMP00000030189 antibody
    • OTTHUMP00000030190 antibody
    • PCNA antibody
    • Pcna/cyclin antibody
    • PCNA_HUMAN antibody
    • PCNAR antibody
    • Polymerase delta accessory protein antibody
    • Proliferating cell nuclear antigen antibody
    • wu:fa28e03 antibody
    • wu:fb36g03 antibody
    see all

Anti-PCNA antibody (Biotin) images

  • All lanes : Anti-PCNA antibody (Biotin) (ab113270) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    developed using the ECL technique

    Predicted band size : 29 kDa

    Exposure time : 3 seconds

References for Anti-PCNA antibody (Biotin) (ab113270)

ab113270 has not yet been referenced specifically in any publications.

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What is the unconjugated version of the biotinylated antibody ab113270?