Overview

  • Product nameAnti-PCNA antibody [EPR3822]
    See all PCNA primary antibodies
  • Description
    Rabbit monoclonal [EPR3822] to PCNA
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human PCNA.

  • Positive control
    • HeLa, HepG2 and 293T cell lysates; Human colonic adenocarcinoma tissue; Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
  • Application notesFlow Cyt: 1/10.
    IHC-P: 1/50 - 1/100. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.

    Is unsuitable for ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
    • Sequence similaritiesBelongs to the PCNA family.
    • Post-translational
      modifications
      Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
      Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
    • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATLD2 antibody
      • cb16 antibody
      • Cyclin antibody
      • DNA polymerase delta auxiliary protein antibody
      • etID36690.10 antibody
      • fa28e03 antibody
      • fb36g03 antibody
      • HGCN8729 antibody
      • MGC8367 antibody
      • Mutagen-sensitive 209 protein antibody
      • OTTHUMP00000030189 antibody
      • OTTHUMP00000030190 antibody
      • PCNA antibody
      • Pcna/cyclin antibody
      • PCNA_HUMAN antibody
      • PCNAR antibody
      • Polymerase delta accessory protein antibody
      • Proliferating cell nuclear antigen antibody
      • wu:fa28e03 antibody
      • wu:fb36g03 antibody
      see all

    Anti-PCNA antibody [EPR3822] images

    • ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty.
    • ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty.
    • All lanes : Anti-PCNA antibody [EPR3822] (ab92729) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 29 kDa

    References for Anti-PCNA antibody [EPR3822] (ab92729)

    This product has been referenced in:
    • Hamajima N  et al. Increased Protein Stability of CDKN1C Causes a Gain-of-Function Phenotype in Patients with IMAGe Syndrome. PLoS One 8:e75137 (2013). WB ; Human . Read more (PubMed: 24098681) »
    • Hanel ML  et al. Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a dynamic nuclear and sarcomeric protein. Differentiation 81:107-18 (2011). WB . Read more (PubMed: 20970242) »

    See all 2 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"