Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin) (ab93576)

Overview

  • Product name
    Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin)
    See all PCNA primary antibodies
  • Description
    Mouse monoclonal [PC10 (a.k.a. 3F81)] to PCNA (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Protein A-Rat PCNA fusion obtained from pC2T.

  • Positive control
    • Alcohol fixed MOLT-4 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: Use 20 µl (or 0.125 µg) for 105-8 cells in a 100 µl volume per test.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
    • Sequence similarities
      Belongs to the PCNA family.
    • Post-translational
      modifications
      Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
      Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
    • Cellular localization
      Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATLD2 antibody
      • cb16 antibody
      • Cyclin antibody
      • DNA polymerase delta auxiliary protein antibody
      • etID36690.10 antibody
      • fa28e03 antibody
      • fb36g03 antibody
      • HGCN8729 antibody
      • MGC8367 antibody
      • Mutagen-sensitive 209 protein antibody
      • OTTHUMP00000030189 antibody
      • OTTHUMP00000030190 antibody
      • PCNA antibody
      • Pcna/cyclin antibody
      • PCNA_HUMAN antibody
      • PCNAR antibody
      • Polymerase delta accessory protein antibody
      • Proliferating cell nuclear antigen antibody
      • wu:fa28e03 antibody
      • wu:fb36g03 antibody
      see all

    Images

    • Intracellular staining of Molt-4 cells with FITC conjugated anti-Human PCNA (PC10) (left), and ab93576 (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

    References

    ab93576 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 28 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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