Anti-PCNA (phospho Y211) antibody (ab85000)

Overview

  • Product nameAnti-PCNA (phospho Y211) antibody
    See all PCNA primary antibodies
  • Description
    Rabbit polyclonal to PCNA (phospho Y211)
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phosphorylated synthetic peptide, representing a portion of human PCNA around tyrosine 211 (NP_002583.1)

  • Positive control
    • Human breast tissue and human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab85000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.
    Extended incubation with primary antibody (16-24 hours) at room temperature is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
    • Sequence similaritiesBelongs to the PCNA family.
    • Post-translational
      modifications
      Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
      Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
    • Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATLD2 antibody
      • cb16 antibody
      • Cyclin antibody
      • DNA polymerase delta auxiliary protein antibody
      • etID36690.10 antibody
      • fa28e03 antibody
      • fb36g03 antibody
      • HGCN8729 antibody
      • MGC8367 antibody
      • Mutagen-sensitive 209 protein antibody
      • OTTHUMP00000030189 antibody
      • OTTHUMP00000030190 antibody
      • PCNA antibody
      • Pcna/cyclin antibody
      • PCNA_HUMAN antibody
      • PCNAR antibody
      • Polymerase delta accessory protein antibody
      • Proliferating cell nuclear antigen antibody
      • wu:fa28e03 antibody
      • wu:fb36g03 antibody
      see all

    Anti-PCNA (phospho Y211) antibody images

    • ab85000 at 1/250 dilution (incubated for 18 hours at room temperature) staining PCNA in serial sections of human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
      Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).
    • ab85000 at 1/250 dilution (incubated for 18 hours at room temperature) staining PCNA in serial sections of human breast tissue by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
      Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right).

    References for Anti-PCNA (phospho Y211) antibody (ab85000)

    ab85000 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"