Anti-PCPTP1 antibody [T10-AF4H11] (ab88958)

Overview

  • Product nameAnti-PCPTP1 antibody [T10-AF4H11]
    See all PCPTP1 primary antibodies
  • Description
    Mouse monoclonal [T10-AF4H11] to PCPTP1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human PCPTP1 purified from E.coli

  • Positive control
    • Bosc23 cell lysate transfected with Myc/His/PCPTP1 (328-651aa).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.03% Sodium Azide
    Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • ClonalityMonoclonal
  • Clone numberT10-AF4H11
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at a concentration of 1 µg/200 µl pre-cleared cell lysate (at 1mg/ml).
    WB: 1/1000. Predicted molecular weight: 74 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus.
    • Tissue specificityExpressed in brain, placenta, small intestine, stomach, uterus and weakly in the prostate. Isoform alpha has been observed only in the brain. Isoform gamma is expressed in brain, placenta and uterus. Isoform delta is expressed in brain, kidney, placenta, prostate, small intestine and uterus.
    • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • Cellular localizationCell membrane and Cytoplasm > perinuclear region. Locates to the perinuclear areas within the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ch-1PTPase antibody
      • Ch1 PTPase antibody
      • DKFZp781C1038 antibody
      • EC PTP antibody
      • ECPTP antibody
      • FLJ34328 antibody
      • MGC131968 antibody
      • MGC148170 antibody
      • NC PTPCOM1 antibody
      • NC-PTPCOM1 antibody
      • Protein tyrosine phosphatase Cr1PTPase precursor antibody
      • Protein tyrosine phosphatase NC PTPCOM1 antibody
      • Protein tyrosine phosphatase PCPTP1 antibody
      • Protein tyrosine phosphatase receptor type R antibody
      • Protein-tyrosine phosphatase PCPTP1 antibody
      • PTP SL antibody
      • PTPBR7 antibody
      • PTPRQ antibody
      • PTPRR antibody
      • PTPRR_HUMAN antibody
      • PTPSL antibody
      • R-PTP-R antibody
      • Receptor type tyrosine protein phosphatase R antibody
      • Receptor-type tyrosine-protein phosphatase R antibody
      see all

    Anti-PCPTP1 antibody [T10-AF4H11] images

    • All lanes : Anti-PCPTP1 antibody [T10-AF4H11] (ab88958) at 1/1000 dilution

      Lane 1 : Bosc23 cell lysate
      Lane 2 : Bosc23 cell lysate transfected with Myc/His/Tau (1-441aa)
      Lane 3 : Bosc23 cell lysate transfected with Myc/His/PCPTP1 (328-651aa)
      Lane 4 : Bosc23 cell lysate transfected with GFP-Ub+1


      Predicted band size : 74 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-PCPTP1 antibody [T10-AF4H11] (ab88958)

    ab88958 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab88958.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"