The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml.
Use a concentration of 0.25 µg/ml.
Crosstide is a synthetic peptide derived from the N terminal sequence of GSK3 and is a well documented substrate of PKB (AKT) and SGK kinases. pCrosstide is the phosphorylated product of these kinases.