Anti-PCSK9 antibody (ab125251)

Overview

  • Product nameAnti-PCSK9 antibody
    See all PCSK9 primary antibodies
  • Description
    Rabbit polyclonal to PCSK9
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Gorilla
  • Immunogen

    Synthetic peptide within Human PCSK9 aa 163-233. The exact sequence is proprietary.

  • Positive control
    • Whole cell lysates derived from HT-29, Mouse liver and Mouse lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab125251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 74 kDa.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.

Target

  • FunctionMay be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • Tissue specificityExpressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • Involvement in diseaseDefects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • Post-translational
    modifications
    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Anti-PCSK9 antibody images

  • All lanes : Anti-PCSK9 antibody (ab125251) at 1/500 dilution

    Lane 1 : HT29 whole cell lysates
    Lane 2 : Mouse liver whole lysates
    Lane 3 : Mouse lung whole lysates


    Predicted band size : 74 kDa

References for Anti-PCSK9 antibody (ab125251)

ab125251 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK293 stable transfectants)
Total protein in input 48000 cells
Immuno-precipitation step Protein A/G
Specification HEK293 stable transfectants
Username

Meihui Pan

Verified customer

Submitted May 22 2015

The antibody is raised against an immunogen within the range of amino acids 163 - 233 of human PCSK9, which is part of the precursor as well as the active form. The single band detected on the blot of mouse liver was 64 kDa and is assumed to be the act...

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I am waiting for the lab that provides this antibody to tell me where the immunogen is located within the range that is specified on the datasheet, aa 100 - 300. The proprotein is aa 31 - 152, and the mature protein is 153 - 692, using the numbering at...

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Thank you for contacting us and your interest in our products.

The antibodies you are interested in are provided following different purification steps. The ab84041 is provides as concentrated culture supernatant, ab5000 is provides as IgG fr...

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Thank you for contacting us.

The Rabbit polyclonal to PCSK9 (ab125251;http://www.abcam.com/PCSK9-antibody-ab125251.html) which we spoke about earlier is our only product that has been tested in ELISA. It recognizes a region around aa100-300 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"