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Goat polyclonal to PCSK9
Predicted to work with:
Human, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human PCSK9 aa 164-175 (internal sequence) (Cysteine residue).
Database link: Q8NBP7
- WB: HEK-293 cell lysate overexpressing recombinant human PCSK9.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 80-100 kDa (predicted molecular weight: 74 kDa).
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Information by UniProt
- Convertase subtilisin/kexin type 9 preproprotein antibody
- FH3 antibody
- HCHOLA3 antibody
Western blot - Anti-PCSK9 antibody (ab223669)
Lane 1 :
Anti-PCSK9 antibody (ab223669) at 0.5 µg/mlLane 2 :
Anti-PCSK9 antibody (ab223669) at 1 µg/mlLane 3 :
Anti-Myc tag at 1/1000 dilutionLanes 1 & 3 :
HEK-293 (human epithelial cell line from embryonic kidney) cells overexpressing recombinant human PCSK9 with C-terminal Myc tag, whole cell lysateLane 2 :
Mock transfected HEK-293 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.Predicted band size:
74 kDaObserved band size:
80-100 kDa (why is the actual band size different from the predicted?
Primary incubations were for 1 hour
has not yet been referenced specifically in any publications.
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