Overview

  • Product name
  • Description
    Goat polyclonal to PCSK9
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Human, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PCSK9 aa 164-175 (internal sequence) (Cysteine residue).
    Sequence:

    C-PRYRADEYQPPD


    Database link: Q8NBP7

  • Positive control
    • WB: HEK-293 cell lysate overexpressing recombinant human PCSK9.

Properties

Applications

Our Abpromise guarantee covers the use of ab223669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 80-100 kDa (predicted molecular weight: 74 kDa).

Target

  • Function
    May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • Tissue specificity
    Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • Involvement in disease
    Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • Sequence similarities
    Belongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • Post-translational
    modifications
    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Images

  • Lane 1 : Anti-PCSK9 antibody (ab223669) at 0.5 µg/ml
    Lane 2 : Anti-PCSK9 antibody (ab223669) at 1 µg/ml
    Lane 3 : Anti-Myc tag at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cells overexpressing recombinant human PCSK9 with C-terminal Myc tag, whole cell lysate
    Lane 2 : Mock transfected HEK-293 cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cells overexpressing recombinant human PCSK9 with C-terminal Myc tag, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 80-100 kDa (why is the actual band size different from the predicted?)

    Primary incubations were for 1 hour

References

ab223669 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223669.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up