The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 74 kDa.
Use a concentration of 5 µg/ml.
Use a concentration of 2 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
FunctionMay be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificityExpressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in diseaseDefects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similaritiesBelongs to the peptidase S8 family. Contains 1 peptidase S8 domain.
Post-translational modificationsThe soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
ICC/IF image of ab28770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, staining PCSK9 with ab28770 at 4 µg/ml. Staining was detected using HRP. Antigen retrieval was by heat mediation in TRIS/EDTA buffer (pH 9).
Western blot - Anti-PCSK9 antibody (ab28770)
Anti-PCSK9 antibody (ab28770) at 0.3 µg/ml + Human Adipose lysate in RIPA buffer at 35 µg developed using the ECL technique
Predicted band size : 74 kDa
References for Anti-PCSK9 antibody (ab28770)
This product has been referenced in:
Liu M et al. PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult mouse brain. J Lipid Res51:2611-8 (2010).
Read more (PubMed: 20453200) »
Lambert G et al. Plasma PCSK9 Concentrations Correlate with LDL and Total Cholesterol in Diabetic Patients and Are Decreased by Fenofibrate Treatment. Clin Chem : (2008).
Read more (PubMed: 18436719) »