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Rabbit polyclonal to PCSK9
Detects both the zymogen and active forms of PC9. Smaller cleavage products are also detected.
Synthetic peptide corresponding to Human PCSK9 (C terminal). From the propeptide cleavage site. Based on the catalytic domain.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/1000. Predicted molecular weight: 74 kDa. 1/1000 when using colorimetric substrates and 1/5000 for chemiluminescent substrates. (pre-pro-peptide runs at 78-80 kDa, the mature form at 58 kDa)
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Information by UniProt
Convertase subtilisin/kexin type 9 preproprotein antibody
Immunocytochemistry/ Immunofluorescence - PCSK9 antibody (ab52754)
ICC/IF image of ab52754 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52754, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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