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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PCSK9 antibody - Propeptide domain
See all PCSK9 products (8) ...
Description
Rabbit polyclonal to PCSK9 - Propeptide domain
Specificity
ab42085 recognises PCSK9 in teh region corresponding to the propeptide cleave site, and detects both the zymogen and active PCSK9.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the propeptide domain of Human PCSK9. (the amino acid sequence is considered proprietary) (Peptide available as ab44562.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 0.5M Sodium chloride
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Metabolism >> Lipid metabolism
Stem Cells >> Neural Stem Cells >> Intracellular
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Signal Transduction >> Metabolism >> Amino Acids
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Western blot - PCSK9 antibody - Propeptide domain (ab42085)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 - 1/5000. Predicted molecular weights: ~15 and 74 kDa. Recommended starting dilution 1/1000 with colourimetric substrates, 1:5000 with chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
Post-translational
modifications
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ8NBP7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FH3 antibody
- HCHOLA3 antibody
- Hypercholesterolemia autosomal dominant 3 antibody
see all
Database links
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
Anti-PCSK9 antibody - Propeptide domain images:
Western blot - PCSK9 antibody - Propeptide domain (ab42085)
All lanes : Anti-PCSK9 antibody - Propeptide domain (ab42085) at 1/1000 dilution
Lane 1 : PCSK9 Protein at 0.08 µg
Lane 2 : Negative control
Lane 3 : PCSK9 Protein at 0.04 µg
Lane 4 : Negative control
Lane 5 : PCSK9 Protein at 0.02 µg
Lane 6 : Negative control at 0.08 µg
Lane 7 : PCSK9 Protein at 0.01 µg
Predicted band size : 74 kDa
Observed band size : 19,64 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-PCSK9 antibody - Propeptide domain (ab42085)
ab42085 has not yet been referenced specifically in any publications.
Publishing research using ab42085? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"