Overview

  • Product nameAnti-PCTAIRE1 antibody
    See all PCTAIRE1 primary antibodies
  • Description
    Mouse monoclonal to PCTAIRE1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MDRMKKIKRQ LSMTLRGGRG IDKTNGAPEQ IGLDESGGGG GSDPGEAPTR AAPGELRSAR GPLSSAPEIV HEDLKMGSDG , corresponding to amino acids 1-80 of Human PCTAIRE1

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab55553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in signal transduction cascades in terminally differentiated cells.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cdk16 antibody
      • CDK16_HUMAN antibody
      • Cell division protein kinase 16 antibody
      • Cyclin-dependent kinase 16 antibody
      • PCTAIRE antibody
      • PCTAIRE protein kinase 1 antibody
      • PCTAIRE-motif protein kinase 1 antibody
      • PCTAIRE1 antibody
      • PCTGAIRE antibody
      • PCTK1 antibody
      • Serine/threonine-protein kinase PCTAIRE-1 antibody
      see all

    Anti-PCTAIRE1 antibody images



    • Predicted band size : 56 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: PCTAIRE1 knockout HAP1 cell lysate (40 µg)
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab55553 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab55553 was shown to recognize PCTAIRE1  when PCTAIRE1  knockout samples were used, along with additional cross-reactive bands. Wild-type and PCTAIRE1  knockout samples were subjected to SDS-PAGE. Ab55553 and ab181602 (loading control to GAPDH) were diluted at 1 µg/ml  and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.



    • Predicted band size : 56 kDa
      PCTAIRE1 antibody (ab55553) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-PCTAIRE1 antibody (ab55553)

    ab55553 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"