Recombinant
RabMAb

Anti-PCTP antibody [EPR15390] (ab184944)

Overview

  • Product name
    Anti-PCTP antibody [EPR15390]
    See all PCTP primary antibodies
  • Description
    Rabbit monoclonal [EPR15390] to PCTP
  • Tested applications
    Suitable for: IP, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PCTP aa 50-150. The exact sequence is proprietary.
    Database link: Q9UKL6

  • Positive control
    • Human fetal liver and K562 cell lysates, K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab184944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30 - 1/50.
Flow Cyt 1/200. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    Catalyzes the transfer of phosphatidylcholine between membranes. Binds a single lipid molecule.
  • Tissue specificity
    Highest expression in liver, placenta, testis, kidney and heart. Low levels in brain and lung. No expression detected in thymus.
  • Sequence similarities
    Contains 1 START domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • PC TP antibody
    • PC-TP antibody
    • PCTP antibody
    • Phosphatidylcholine transfer protein antibody
    • PPCT_HUMAN antibody
    • StAR related lipid transfer (START) domain containing 2 antibody
    • StAR related lipid transfer protein 2 antibody
    • StAR-related lipid transfer protein 2 antibody
    • STARD 2 antibody
    • StARD2 antibody
    • START domain containing 2 antibody
    • START domain containing protein 2 antibody
    • START domain-containing protein 2 antibody
    see all

Images

  • All lanes : Anti-PCTP antibody [EPR15390] (ab184944) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • Flow cytometry analysis of paraformaldehyde-fixed K562 cells labeling PCTP with ab184944 at a 1/200 dilution (Red), with a negative control rabbit IgG (Green). Secondary antibody goat anti-rabbit IgG (FITC) at a 1/150 dilution.

  • Western blot analysis on immunoprecipitation from Human fetal liver lysate (Lane 1), labeling PCTP using ab184944 at 1/50 dilution Lane 2 shows PBS negative control. Secondary antibody, HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab184944 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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