Overview

  • Product name
  • Description
    Rabbit polyclonal to PCYOX1
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    A region within synthetic peptide: PSTDGTYVWK IFSQETLTKA QILKLFLSYD YAVKKPWLAY PHYKPPEKCP, corresponding to C terminal amino acids 397-446 of Human PCYOX1

  • Positive control
    • Jurkat cell lysate and human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab49347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the prenylcysteine oxidase family.
  • Post-translational
    modifications
    The protein is glycosylated at one or more potential N-glycosylation sites.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 1.8.3.5 antibody
    • KIAA0908 antibody
    • PCL1 antibody
    • PCYOX_HUMAN antibody
    • PCYOX1 antibody
    • Prenylcysteine lyase antibody
    • Prenylcysteine oxidase 1 antibody
    • Prenylcysteine oxidase precursor antibody
    see all

Images

  • Anti-PCYOX1 antibody (ab49347) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000

    Predicted band size : 57 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • This image shows human liver tissue stained with ab49347 at 4µg/ml. Positive label (arrows) are hematopoietic cells. Magnification X400.

References

ab49347 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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