Overview

  • Product name
    Anti-PCYOX1 antibody
    See all PCYOX1 primary antibodies
  • Description
    Rabbit polyclonal to PCYOX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    A region within synthetic peptide: PSTDGTYVWK IFSQETLTKA QILKLFLSYD YAVKKPWLAY PHYKPPEKCP, corresponding to C terminal amino acids 397-446 of Human PCYOX1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Jurkat cell lysate and human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab49347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the prenylcysteine oxidase family.
  • Post-translational
    modifications
    The protein is glycosylated at one or more potential N-glycosylation sites.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
    • Alternative names
      • EC 1.8.3.5 antibody
      • KIAA0908 antibody
      • PCL1 antibody
      • PCYOX_HUMAN antibody
      • PCYOX1 antibody
      • Prenylcysteine lyase antibody
      • Prenylcysteine oxidase 1 antibody
      • Prenylcysteine oxidase precursor antibody
      see all

    Images

    • Western blot - PCYOX1 antibody (ab49347)
      Western blot - PCYOX1 antibody (ab49347)
      Anti-PCYOX1 antibody (ab49347) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000

      Predicted band size: 57 kDa
      Observed band size: 58 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Paraffin-embedded sections) - PCYOX1 antibody (ab49347)
      Immunohistochemistry (Paraffin-embedded sections) - PCYOX1 antibody (ab49347)
      This image shows human liver tissue stained with ab49347 at 4µg/ml. Positive label (arrows) are hematopoietic cells. Magnification X400.

    References

    ab49347 has not yet been referenced specifically in any publications.

    Publishing research using ab49347? Please let us know so that we can cite the reference in this datasheet.

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