Overview

  • Product name
    Anti-PCYT1A antibody [EPR3940(2)]
    See all PCYT1A primary antibodies
  • Description
    Rabbit monoclonal [EPR3940(2)] to PCYT1A
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PCYT1A aa 1-100. The exact sequence is proprietary.
    Database link: P49585

  • Positive control
    • WB: HepG2, HeLa, A549, and K562 cell lysates IHC-P: Human colon and Human prostatic hyperplasia tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Controls phosphatidylcholine synthesis.
    • Pathway
      Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
    • Sequence similarities
      Belongs to the cytidylyltransferase family.
    • Post-translational
      modifications
      The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
    • Cellular localization
      Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCT A antibody
      • CCT alpha antibody
      • CCT-alpha antibody
      • Choline phosphate cytidylyltransferase A antibody
      • Choline-phosphate cytidylyltransferase A antibody
      • CT A antibody
      • CT antibody
      • CTP:phosphocholine cytidylyltransferase A antibody
      • CTPCT antibody
      • PCY1A_HUMAN antibody
      • PCYT1 antibody
      • Pcyt1a antibody
      • Phosphate cytidylyltransferase 1 choline alpha antibody
      • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
      • Phosphorylcholine transferase A antibody
      see all

    Images

    • All lanes : Anti-PCYT1A antibody [EPR3940(2)] (ab109263) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • ab109263, at a 1/50 dilution, staining PCYT1A in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ab109263, at a 1/50 dilution, staining PCYT1A in paraffin embedded Human prostatic hyperplasia by Immunohistochemistry.

    References

    This product has been referenced in:
    • Payne F  et al. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A 111:8901-6 (2014). WB ; Human . Read more (PubMed: 24889630) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109263.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up