1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionControls phosphatidylcholine synthesis.
PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
Sequence similaritiesBelongs to the cytidylyltransferase family.
Post-translational modificationsThe serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
Cellular localizationCytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
ab109263, at a 1/50 dilution, staining PCYT1A in paraffin embedded Human prostatic hyperplasia by Immunohistochemistry.
References for Anti-PCYT1A antibody [EPR3940(2)] (ab109263)
This product has been referenced in:
Payne F et al. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A111:8901-6 (2014).
Read more (PubMed: 24889630) »