Overview

  • Product nameAnti-PCYT1A antibody [EPR3940(2)]
    See all PCYT1A primary antibodies
  • Description
    Rabbit monoclonal [EPR3940(2)] to PCYT1A
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PCYT1A aa 1-100. The exact sequence is proprietary.
    Database link: P49585

  • Positive control
    • WB: HepG2, HeLa, A549, and K562 cell lysates IHC-P: Human colon and Human prostatic hyperplasia tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionControls phosphatidylcholine synthesis.
    • PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
    • Sequence similaritiesBelongs to the cytidylyltransferase family.
    • Post-translational
      modifications
      The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
    • Cellular localizationCytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCT A antibody
      • CCT alpha antibody
      • CCT-alpha antibody
      • Choline phosphate cytidylyltransferase A antibody
      • Choline-phosphate cytidylyltransferase A antibody
      • CT A antibody
      • CT antibody
      • CTP:phosphocholine cytidylyltransferase A antibody
      • CTPCT antibody
      • PCY1A_HUMAN antibody
      • PCYT1 antibody
      • Pcyt1a antibody
      • Phosphate cytidylyltransferase 1 choline alpha antibody
      • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
      • Phosphorylcholine transferase A antibody
      see all

    Anti-PCYT1A antibody [EPR3940(2)] images

    • All lanes : Anti-PCYT1A antibody [EPR3940(2)] (ab109263) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • ab109263, at a 1/50 dilution, staining PCYT1A in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ab109263, at a 1/50 dilution, staining PCYT1A in paraffin embedded Human prostatic hyperplasia by Immunohistochemistry.

    References for Anti-PCYT1A antibody [EPR3940(2)] (ab109263)

    This product has been referenced in:
    • Payne F  et al. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A 111:8901-6 (2014). WB ; Human . Read more (PubMed: 24889630) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab109263.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"