Recombinant
RabMAb

Anti-PCYT1A antibody [EPR3941] (ab92440)

Overview

  • Product name
    Anti-PCYT1A antibody [EPR3941]
    See all PCYT1A primary antibodies
  • Description
    Rabbit monoclonal [EPR3941] to PCYT1A
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PCYT1A (UniProt ID: P49585).

  • Positive control
    • A549, K562, HeLa and HepG2 cell lysates; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
ICC/IF
  • Application notes
    Flow Cyt: 1/500 - 1/1000.
    ICC/IF: 1/100 - 1/250.
    IP: 1/10 - 1/100.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.

    Is unsuitable for IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Controls phosphatidylcholine synthesis.
    • Pathway
      Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
    • Sequence similarities
      Belongs to the cytidylyltransferase family.
    • Post-translational
      modifications
      The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
    • Cellular localization
      Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCT A antibody
      • CCT alpha antibody
      • CCT-alpha antibody
      • Choline phosphate cytidylyltransferase A antibody
      • Choline-phosphate cytidylyltransferase A antibody
      • CT A antibody
      • CT antibody
      • CTP:phosphocholine cytidylyltransferase A antibody
      • CTPCT antibody
      • PCY1A_HUMAN antibody
      • PCYT1 antibody
      • Pcyt1a antibody
      • Phosphate cytidylyltransferase 1 choline alpha antibody
      • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
      • Phosphorylcholine transferase A antibody
      see all

    Images

    • All lanes : Anti-PCYT1A antibody [EPR3941] (ab92440) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
    • ab92440, at 1/100 dilution, staining PCYT1A in HeLa cells by Immunofluorescence.

    References

    ab92440 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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