Recombinant
RabMAb

Anti-PCYT1A antibody [EPR3941] (ab92440)

Abreviews (1) Submit a Question

Overview

  • Product name
    Anti-PCYT1A antibody [EPR3941]
    See all PCYT1A primary antibodies
  • Description
    Rabbit monoclonal [EPR3941] to PCYT1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PCYT1A (UniProt ID: P49585).

  • Positive control
    • A549, K562, HeLa and HepG2 cell lysates; HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
Flow Cyt
ICC/IF
  • Application notes
    Flow Cyt: 1/500 - 1/1000.
    ICC/IF: 1/100 - 1/250.
    IP: 1/10 - 1/100.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.

    Is unsuitable for IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Controls phosphatidylcholine synthesis.
    • Pathway
      Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
    • Sequence similarities
      Belongs to the cytidylyltransferase family.
    • Post-translational
      modifications
      The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
    • Cellular localization
      Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
    • Information by UniProt
    • Database links
      • Alternative names
        • CCT A antibody
        • CCT alpha antibody
        • CCT-alpha antibody
        • Choline phosphate cytidylyltransferase A antibody
        • Choline-phosphate cytidylyltransferase A antibody
        • CT A antibody
        • CT antibody
        • CTP:phosphocholine cytidylyltransferase A antibody
        • CTPCT antibody
        • PCY1A_HUMAN antibody
        • PCYT1 antibody
        • Pcyt1a antibody
        • Phosphate cytidylyltransferase 1 choline alpha antibody
        • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
        • Phosphorylcholine transferase A antibody
        see all

      Images

      • Western blot - Anti-PCYT1A antibody [EPR3941] (ab92440)
        Western blot - Anti-PCYT1A antibody [EPR3941] (ab92440)
        All lanes : Anti-PCYT1A antibody [EPR3941] (ab92440) at 1/1000 dilution

        Lane 1 : A549 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 42 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PCYT1A antibody [EPR3941] (ab92440)
        Immunocytochemistry/ Immunofluorescence - Anti-PCYT1A antibody [EPR3941] (ab92440)
        ab92440, at 1/100 dilution, staining PCYT1A in HeLa cells by Immunofluorescence.

      References

      ab92440 has not yet been referenced specifically in any publications.

      Publishing research using ab92440? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot

       Average
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Human Cell lysate - whole cell (U2OS)
      Specification
      U2OS
      Blocking step
      Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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      Verified customer

      Submitted Jun 05 2013

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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