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Rabbit monoclonal [EPR3941] to PCYT1A
ICC/IF more details Unsuitable for:
Synthetic peptide corresponding to residues in Human PCYT1A (UniProt ID: P49585).
A549, K562, HeLa and HepG2 cell lysates; HeLa cells
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/500 - 1/1000.
ICC/IF: 1/100 - 1/250.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Controls phosphatidylcholine synthesis.
Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
Belongs to the cytidylyltransferase family.
The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.
Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
Information by UniProt
CCT A antibody
CCT alpha antibody
Western blot - PCYT1A antibody [EPR3941] (ab92440)
All lanes : Anti-PCYT1A antibody [EPR3941] (ab92440) at 1/1000 dilution Lane 1 : A549 cell lysate Lane 2 : K562 cell lysate Lane 3 : HeLa cell lysate Lane 4 : HepG2 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 42 kDa
Immunocytochemistry/ Immunofluorescence - PCYT1A antibody [EPR3941] (ab92440)
ab92440, at 1/100 dilution, staining PCYT1A in HeLa cells by Immunofluorescence.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"