- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PCYT1A antibody [EPR3941]
See all PCYT1A primary antibodies -
DescriptionRabbit monoclonal [EPR3941] to PCYT1A
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Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to residues in Human PCYT1A (UniProt ID: P49585).
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Positive control
- A549, K562, HeLa and HepG2 cell lysates; HeLa cells
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General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3941
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab92440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| Flow Cyt | ||
| ICC/IF |
ICC/IF: 1/100 - 1/250.
IP: 1/10 - 1/100.
WB: 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
Is unsuitable for IHC-P.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionControls phosphatidylcholine synthesis.
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PathwayPhospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.
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Sequence similaritiesBelongs to the cytidylyltransferase family.
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Post-translational
modificationsThe serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation. -
Cellular localizationCytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
- Information by UniProt
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Database links
- Entrez Gene: 5130 Human
- Omim: 123695 Human
- SwissProt: P49585 Human
- Unigene: 135997 Human
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Alternative names
- CCT A antibody
- CCT alpha antibody
- CCT-alpha antibody
see all
Anti-PCYT1A antibody [EPR3941] images
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![Western blot - PCYT1A antibody [EPR3941] (ab92440)](http://a.abcam.com/ps/datasheet/images/92/ab92440/PCYT1A-Primary-antibodies-ab92440-3.jpg)
Western blot - PCYT1A antibody [EPR3941] (ab92440)All lanes : Anti-PCYT1A antibody [EPR3941] (ab92440) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 42 kDa -
![Immunocytochemistry/ Immunofluorescence - PCYT1A antibody [EPR3941] (ab92440)](http://a.abcam.com/ps/datasheet/images/92/ab92440/PCYT1A-Primary-antibodies-ab92440-2.jpg)
Immunocytochemistry/ Immunofluorescence - PCYT1A antibody [EPR3941] (ab92440)ab92440, at 1/100 dilution, staining PCYT1A in HeLa cells by Immunofluorescence.
Protocols
References for Anti-PCYT1A antibody [EPR3941] (ab92440)
ab92440 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"