Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (NSQ-NSCLC)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EnVision FLEX TRS, Low pH
Permeabilization No
Specification NSQ-NSCLC
Blocking step EnV FLEX Peroxidase-Blocking Reagent as blocking agent for 3 minute(s) · Concentration: 100%
Fixative Formaldehyde

Other product details

Incubation time 1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution 1/400

Secondary antibody

Secondary antibody None used

Additional data

Additional Notes This protocol used EnVision Flex Reagents for detection on the Dako Omnis automated stainer. The Secondary Reagent used was the EnV FLEX + Rabbit Linker followed by the EnV FLEX/HRP Polymer.

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Submitted May 11 2016