• Product nameAnti-PD-L1 antibody [MIH5] (Biotin)
    See all PD-L1 primary antibodies
  • Description
    Rat monoclonal [MIH5] to PD-L1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse PD-L1. DBA/2 Mouse lymphoma L5178Y transfected with PL-L1 cDNA

  • Positive control
    • Mouse C57Bl/6 splenocytes
  • General notes

    ab95792 is reported to be a blocking antibody.



Our Abpromise guarantee covers the use of ab95792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.25µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.

ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane and Endomembrane system.
  • Information by UniProt
  • Database links
  • Alternative names
    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

Anti-PD-L1 antibody [MIH5] (Biotin) images

  • Flow cytometric staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 µg of ab95792 (filled histogram), followed by Streptavidin PE. Total viable cells were used for analysis.

References for Anti-PD-L1 antibody [MIH5] (Biotin) (ab95792)

This product has been referenced in:
  • Sheng H  et al. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res 18:846-57 (2008). Flow Cyt . Read more (PubMed: 18607390) »
  • Saudemont A  et al. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood 105:2428-35 (2005). Read more (PubMed: 15536145) »

See all 3 Publications for this product

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