The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125-0.25µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
Flow cytometric staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Biotin (open histogram) or 0.125 µg of ab95792 (filled histogram), followed by Streptavidin PE. Total viable cells were used for analysis.
References for Anti-PD-L1 antibody [MIH5] (Biotin) (ab95792)
This product has been referenced in:
Sheng H et al. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res18:846-57 (2008).
Read more (PubMed: 18607390) »
Saudemont A et al. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood105:2428-35 (2005).
Read more (PubMed: 15536145) »