Recombinant
RabMAb

Anti-PD-L2 antibody [EPR1163(2)] (ab187662)

Overview

  • Product name
    Anti-PD-L2 antibody [EPR1163(2)]
    See all PD-L2 primary antibodies
  • Description
    Rabbit monoclonal [EPR1163(2)] to PD-L2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PD-L2 aa 50-150. The exact sequence is proprietary.
    Database link: Q9BQ51

  • Positive control
    • Raji, HepG2, THP-1, C6, Raw264.7, PC-12 and NIH 3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR1163(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

  • Function
    Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
  • Tissue specificity
    Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted; Cell membrane and Endomembrane system.
  • Information by UniProt
  • Database links
  • Alternative names
    • B7 dendritic cell molecule antibody
    • B7-DC antibody
    • B7DC antibody
    • bA574F11.2 antibody
    • Btdc antibody
    • Butyrophilin B7 DC antibody
    • Butyrophilin B7-DC antibody
    • Butyrophilin B7DC antibody
    • CD 273 antibody
    • CD273 antibody
    • CD273 antigen antibody
    • MGC142238 antibody
    • MGC142240 antibody
    • PD 1 ligand 2 antibody
    • PD L2 antibody
    • PD-1 ligand 2 antibody
    • PD-L2 antibody
    • PD1 ligand 2 antibody
    • PD1L2_HUMAN antibody
    • PDCD 1 ligand 2 antibody
    • PDCD1 ligand 2 antibody
    • PDCD1L2 antibody
    • Pdcd1lg2 antibody
    • PDL 2 antibody
    • PDL2 antibody
    • Programmed cell death 1 ligand 2 antibody
    • Programmed death ligand 2 antibody
    see all

Images

  • All lanes : Anti-PD-L2 antibody [EPR1163(2)] (ab187662) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • All lanes : Anti-PD-L2 antibody [EPR1163(2)] (ab187662) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

References

This product has been referenced in:
  • Zheng B  et al. PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse. J Hematol Oncol 11:16 (2018). WB . Read more (PubMed: 29409495) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Placenta
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 12 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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