The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.
Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.