The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06-0.5µg for 105-8 cells.
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.
Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Staining of non-transfected (left) and mouse PD-L2 transfected (right) cells with 0.25µg PE Rat IgG2a isotype control (open histogram) or 0.06µg ab93589 (colored histogram). Total viable cells were used for analysis.