The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.06-0.5µg for 105-8 cells.
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
Tissue specificityHighly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted; Cell membrane and Endomembrane system.
Staining of non-transfected (left) and mouse PD-L2 transfected (right) cells with 0.25µg PE Rat IgG2a isotype control (open histogram) or 0.06µg ab93589 (colored histogram). Total viable cells were used for analysis.
References for Anti-PD-L2 antibody [TY25] (Phycoerythrin) (ab93589)
This product has been referenced in:
Tanaka K et al. PDL1 is required for peripheral transplantation tolerance and protection from chronic allograft rejection. J Immunol179:5204-10 (2007).
Read more (PubMed: 17911605) »