Overview

  • Product name
    Anti-PD1 antibody [J116]
    See all PD1 primary antibodies
  • Description
    Mouse monoclonal [J116] to PD1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human PD1 protein

  • Positive control
    • Human tonsil tissue; Human PD1 transfected cells

Properties

Applications

Our Abpromise guarantee covers the use of ab140950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 105-8 cells.

(In a final volume of 100 µl).

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Possible cell death inducer, in association with other factors.
  • Involvement in disease
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    Induced at programmed cell death.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

Images

  • ab140950 staining PD1 in human atopic dermatitis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (20μg/ml) for 16 hours at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (10μg/ml) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labelling PD1 with ab140950 at 20 µg/ml.
  • Flow Cytometry analysis of peripheral blood cells, separated by Ficoll density separation labeling PD1 with ab140950 at 1µg/ml. PBS with human IgG (Fc-block) was used as the sample buffer. A goat anti-mouse FITC conjugated secondary antibody was used at 1/100 dilution. Isotype control (1), Untreated control (2), PHA treated (5ng/ml) (3).

    See Abreview

  • ab140950 staining PD1 in human nasopharynx tissue sections by Immunohistochemistry (Formalin/PFA-fixed frozen paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer (pH 6.0). The sample was incubated with primary antibody (20ug/ml) in blocking buffer at 4°C for 16 hours. An Alexa Fluor® 488-conjugated Goat anti-mouse polyclonal (1/100) was used as the secondary antibody.

    See Abreview

References

ab140950 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

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Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin (atopic dermatitis))
Permeabilization
No
Specification
skin (atopic dermatitis)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Methanol
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Submitted Nov 24 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (nasopharynx)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
No
Specification
nasopharynx
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Nov 24 2015

Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Permeabilization
No
Gating Strategy
lympho and mono gate
Specification
PBMCs
Preparation
Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation
Sample buffer: PBS with human IgG (Fc-block)
Fixation
no fixation
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Submitted Sep 25 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (PBMCs)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Loading amount
15 µg
Treatment
see below
Specification
PBMCs
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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