Anti-PD1 antibody [J43] (ab95789)
Key features and details
- Armenian hamster monoclonal [J43] to PD1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-PD1 antibody [J43]
See all PD1 primary antibodies -
Description
Armenian hamster monoclonal [J43] to PD1 -
Host species
Armenian hamster -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse PD1. Syrian Hamster kidney cell line BKH transfected with Mouse PD1 cDNA.
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Positive control
- 3-day ConA activated BALB/c Mouse splenocytes.
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General notes
It is reported that monoclonal antibody J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
It is reported that monoclonal antibody J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2. -
Clonality
Monoclonal -
Clone number
J43 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.5µg for 105-8 cells.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 0.5µg for 105-8 cells. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 18566 Mouse
- SwissProt: Q02242 Mouse
- Unigene: 5024 Mouse
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Alternative names
- CD279 antibody
- CD279 antigen antibody
- hPD 1 antibody
see all
Images
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Flow cytometry staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c Mouse splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control (open histogram) or 0.25 µg of ab95789 (filled histogram) followed by Anti-Armenian Hamster IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
Datasheets and documents
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Datasheet download
References (3)
ab95789 has been referenced in 3 publications.
- Sahyon HA & Al-Harbi SA Chemoprotective role of an extract of the heart of the Phoenix dactylifera tree on adriamycin-induced cardiotoxicity and nephrotoxicity by regulating apoptosis, oxidative stress and PD-1 suppression. Food Chem Toxicol 135:111045 (2020). PubMed: 31830546
- Ansari MJ et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J Exp Med 198:63-9 (2003). PubMed: 12847137
- Salama AD et al. Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis. J Exp Med 198:71-8 (2003). PubMed: 12847138