The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 0.5µg for 105-8 cells.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Possible cell death inducer, in association with other factors.
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Flow cytometric analysis of 3-day unstimulated (left panel) and 3-day ConA-stimulated (right panel) BALB/c splenocytes with 0.25 µg of ab171266 (filled histogram) with Anti-Mouse CD279 (PD1) Functional Grade Purified antibody (open histogram) followed by Anti-Armenian Hamster IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.