Anti-PD1 antibody [MIH4] (FITC) (ab110177)

Overview

  • Product name
    Anti-PD1 antibody [MIH4] (FITC)
    See all PD1 primary antibodies
  • Description
    Mouse monoclonal [MIH4] to PD1 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Human CD279 - transfected L cells

Properties

Applications

Our Abpromise guarantee covers the use of ab110177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/10. Use 10µl of the suggested working dilution to label 1x106 cells in 100µl



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Possible cell death inducer, in association with other factors.
  • Involvement in disease
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    Induced at programmed cell death.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

References for Anti-PD1 antibody [MIH4] (FITC) (ab110177)

ab110177 has not yet been referenced specifically in any publications.

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Read More
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 08 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (from buffy coat)
Specification
from buffy coat
Preparation
Cell harvesting/tissue preparation method: after ficoll purification
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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