Overview

  • Product name
    Anti-PD1 antibody [RMP1-14]
    See all PD1 primary antibodies
  • Description
    Rat monoclonal [RMP1-14] to PD1
  • Tested applications
    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    PDCD1 transfected BHK cells.

  • General notes


    Antibodies produced by clone RMP1-14 have been reported to block the binding of both B7-H1-Ig and B7-DC-Ig fusion proteins to PDCD1 transfected BHK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Formaldehyde-based fixatives such as PFA are not recommended. Fix frozen, unfixed tissue sections on the slide with ice-cold acetone for 15-20 minutes.

Flow Cyt 1/10 - 1/25. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Possible cell death inducer, in association with other factors.
  • Involvement in disease
    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage
    Induced at programmed cell death.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD279 antibody
    • CD279 antigen antibody
    • hPD 1 antibody
    • hPD l antibody
    • hPD-1 antibody
    • hSLE1 antibody
    • PD 1 antibody
    • PD-1 antibody
    • PD1 antibody
    • PDCD 1 antibody
    • PDCD1 antibody
    • PDCD1_HUMAN antibody
    • Programmed cell death 1 antibody
    • Programmed cell death 1 protein antibody
    • Programmed cell death protein 1 antibody
    • Protein PD 1 antibody
    • Protein PD-1 antibody
    • SLEB2 antibody
    • Systemic lupus erythematosus susceptibility 2 antibody
    see all

References for Anti-PD1 antibody [RMP1-14] (ab63477)

This product has been referenced in:
  • Gray MJ  et al. Phosphatidylserine-targeting antibodies augment the anti-tumorigenic activity of anti-PD-1 therapy by enhancing immune activation and downregulating pro-oncogenic factors induced by T-cell checkpoint inhibition in murine triple-negative breast cancers. Breast Cancer Res 18:50 (2016). Read more (PubMed: 27169467) »
  • Ehlers M  et al. Immunoregulatory natural killer cells suppress autoimmunity by down-regulating antigen-specific CD8+ T cells in mice. Endocrinology 153:4367-79 (2012). Read more (PubMed: 22733969) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (lymphocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 05 2017

The amino acid sequence of the antibody itself has not been determined, to our knowledge.

If you need the sequence of the immunogen that generated the antibody, the immunogen and immunization procedure are described in a paper by the laborator...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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