PDCD1 transfected BHK cells.
Antibodies produced by clone RMP1-14 have been reported to block the binding of both B7-H1-Ig and B7-DC-Ig fusion proteins to PDCD1 transfected BHK cells.
Our Abpromise guarantee covers the use of ab63477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.
Formaldehyde-based fixatives such as PFA are not recommended. Fix frozen, unfixed tissue sections on the slide with ice-cold acetone for 15-20 minutes.
|Flow Cyt||1/10 - 1/25. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|