Overview

  • Product nameAnti-PDCD10 antibody
    See all PDCD10 primary antibodies
  • Description
    Rabbit polyclonal to PDCD10
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Chimpanzee, Macaque Monkey, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PDCD10.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HeLa; Jurkat; K562; SAOS2; MCF7. It also gave a positive signal in FFPE human kidney RCC tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab110531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 25 kDa).

Target

  • FunctionPromotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:603285]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.
  • Sequence similaritiesBelongs to the PDCD10 family.
  • Cellular localizationCytoplasm. Golgi apparatus membrane. Cell membrane. Partially co-localizes with endogenous PXN at the leading edges of migrating cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis related protein 15 antibody
    • CCM3 antibody
    • Cerebral cavernous malformations 3 protein antibody
    • MGC1212 antibody
    • MGC24477 antibody
    • PDC10_HUMAN antibody
    • PDCD 10 antibody
    • PDCD10 antibody
    • Programmed cell death 10 antibody
    • Programmed cell death protein 10 antibody
    • TF 1 cell apoptosis related protein 15 antibody
    • TF-1 cell apoptosis-related protein 15 antibody
    • TFAR15 antibody
    see all

Anti-PDCD10 antibody images

  • All lanes : Anti-PDCD10 antibody (ab110531) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 5 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of ab110531 staining in human kidney RCC formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110531, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PDCD10 antibody (ab110531)

ab110531 has not yet been referenced specifically in any publications.

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