Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998)
Key features and details
- Mouse monoclonal [9G6] to PDCD4 (phospho S457)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-PDCD4 (phospho S457) antibody [9G6]
See all PDCD4 primary antibodies -
Description
Mouse monoclonal [9G6] to PDCD4 (phospho S457) -
Host species
Mouse -
Specificity
ab105998 is specific for Human PDCD4 protein phosphorylated at Ser457. Cross-reactivity with PDCD4 from other sources has not been determined. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis -
Immunogen
Synthetic peptide corresponding to Human PDCD4 (phospho S457).
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Positive control
- NIH-3T3 cells stimulated with PDGF, Recombinant PDCD4 protein, Human small intestine tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from concentrated tissue culture supernate by Protein A chromatography. -
Clonality
Monoclonal -
Clone number
9G6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/1000 - 1/5000.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 62 kDa. |
IHC-P
1/1000 - 1/5000. |
Target
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Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. -
Tissue specificity
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. -
Sequence similarities
Belongs to the PDCD4 family.
Contains 2 MI domains. -
Domain
Binds EIF4A1 via both MI domains. -
Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. - Information by UniProt
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Database links
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 711490 Human
see all -
Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Images
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All lanes : Anti-PDCD4 (phospho S457) antibody [9G6] (ab105998) at 1/2000 dilution
Lane 1 : Unstimulated (non-phosphorylated) NIH 3T3 cells
Lane 2 : NIH-3T3 cells stimulated with 30 ng/ml PDGF (5 min treatment)
Secondary
All lanes : HRP conjugated Rabbit anti-Mouse IgG at 1/20000 dilution
Predicted band size: 62 kDa -
ab105998 at 1/1000 dilution staining PDCD4 in Human small intestine by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows the localization of ab105998 as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab105998 has been referenced in 3 publications.
- Kenawy N et al. Conjunctival melanoma copy number alterations and correlation with mutation status, tumor features, and clinical outcome. Pigment Cell Melanoma Res 32:564-575 (2019). PubMed: 30672666
- Yuan H et al. Downregulation of PDCD4 by miR-21 suppresses tumor transformation and proliferation in a nude mouse renal cancer model. Oncol Lett 14:3371-3378 (2017). PubMed: 28927090
- Mao XH et al. MicroRNA-21 regulates the ERK/NF-?B signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN. Mol Carcinog 56:886-894 (2017). PubMed: 27533779