Anti-PDCD4 (phospho S457) antibody (ab118505)

Overview

  • Product name
    Anti-PDCD4 (phospho S457) antibody
    See all PDCD4 primary antibodies
  • Description
    Rabbit polyclonal to PDCD4 (phospho S457)
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Orangutan
  • Immunogen

    Synthetic phosphopeptide: CPSRGRKRFVSPEGDGGRLK conjugated to KLH, corresponding to amino acids 447-465 of Human PDCD4

  • Positive control
    • Human breast tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab118505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/5000. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 1.25 - 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1500 - 1/7000.

Target

  • Function
    Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificity
    Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similarities
    Belongs to the PDCD4 family.
    Contains 2 MI domains.
  • Domain
    Binds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Images

  • ab118505, at 2.5 µg/ml, staining PDCD4 in formalin-fixed, paraffin-embedded Human Breast tissue by Immunohistochemistry using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab118505 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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