Anti-PDCD4 (phospho S67) antibody (ab73343)
Key features and details
- Rabbit polyclonal to PDCD4 (phospho S67)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDCD4 (phospho S67) antibody
See all PDCD4 primary antibodies -
Description
Rabbit polyclonal to PDCD4 (phospho S67) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human PDCD4 (phospho S67).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab73343 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. -
Tissue specificity
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. -
Sequence similarities
Belongs to the PDCD4 family.
Contains 2 MI domains. -
Domain
Binds EIF4A1 via both MI domains. -
Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. - Information by UniProt
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Database links
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 711490 Human
see all -
Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab73343 has been referenced in 5 publications.
- Li C et al. SKP2 promotes breast cancer tumorigenesis and radiation tolerance through PDCD4 ubiquitination. J Exp Clin Cancer Res 38:76 (2019). PubMed: 30760284
- Kroczynska B et al. Interferon ? (IFN?) Signaling via Mechanistic Target of Rapamycin Complex 2 (mTORC2) and Regulatory Effects in the Generation of Type II Interferon Biological Responses. J Biol Chem 291:2389-96 (2016). PubMed: 26645692
- Ge C et al. Atheroprotective pulsatile flow induces ubiquitin-proteasome-mediated degradation of programmed cell death 4 in endothelial cells. PLoS One 9:e91564 (2014). PubMed: 24626527
- Schmid T et al. Inflammation-induced loss of Pdcd4 is mediated by phosphorylation-dependent degradation. Carcinogenesis 32:1427-33 (2011). PubMed: 21771721
- Wang X et al. US3 protein kinase of HSV-1 cycles between the cytoplasm and nucleus and interacts with programmed cell death protein 4 (PDCD4) to block apoptosis. Proc Natl Acad Sci U S A 108:14632-6 (2011). PubMed: 21844356