Anti-PDCD5 antibody (ab83958)

Overview

  • Product name
  • Description
    Rabbit polyclonal to PDCD5
  • Tested applications
    Suitable for: ICC, ICC/IF, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 21 amino acid synthetic peptide from near the carboxy terminus of Human PDCD5.

  • Positive control
    • EL4 cell lysate. Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab83958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

Images

  • Anti-PDCD5 antibody (ab83958) at 1 µg/ml + EL4 cell lysate at 15 µg

    Predicted band size : 14 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry of PDCD5 in Jurkat cells using ab83958 at 2ug/ml.
  • Immunofluorescence of PDCD5 in Jurkat cells using ab83958 at 10 ug/ml.

References

ab83958 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Tissue lysate - nuclear (HEK 293 T)
Total protein in input
100 µg
Specification
HEK 293 T
Immuno-precipitation step
Protein A/G
Username

Dr. Chris Tracy

Verified customer

Submitted Sep 26 2012

DISCOUNT CODE: XXXXX Expiration date: XX-XX-XX   I am very pleased to hear you would like to accept our offer and test ab83958 in ChIP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, p...

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Application
Immunoprecipitation
Sample
Rabbit Cell lysate - whole cell (HEK 293T and U2OS)
Total protein in input
1000 µg
Specification
HEK 293T and U2OS
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Jun 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
10 µg
Specification
HEK 293T
Treatment
Negative Control or PDCD5 siRNA for 96 hrs
Gel Running Conditions
Reduced Denaturing (16.5% acrylamide, tricine)
Blocking step
LiCor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 Cells)
Loading amount
10 µg
Specification
HEK293 Cells
Gel Running Conditions
Reduced Denaturing (16% Tricine)
Blocking step
Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

Submitted May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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