Overview

  • Product nameAnti-Pdd1 antibody - ChIP Grade
  • Description
    Rabbit polyclonal to Pdd1 - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ChIPmore details
  • Species reactivity
    Reacts with: Tetrahymena
  • Immunogen

    Synthetic peptide derived from residues 300 - 400 of Tetrahymena sp. Pdd1.

    .

Properties

Applications

Our Abpromise guarantee covers the use of ab5338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 57 kDa).
IP Use at an assay dependent dilution.
ChIP Use at an assay dependent concentration. PubMed: 20357003

Target

  • RelevanceThe PDD1 gene sequence predicts a novel polypeptide containing two copies of a chromodomain, a conserved domain found in a number of heterochromatin-associated proteins (Koonin et al. 1995). Consistent with this finding, Pdd1p localizes to electron-dense heterochromatic structures that concentrate at the periphery of macronuclear anlagen during the time when DNA deletion occurs.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • p65 antibody
      • Pdd 1 antibody
      • Pdd 1p antibody
      • Pdd1p antibody
      • Programmed DNA degradation (pdd) antibody
      see all

    Anti-Pdd1 antibody - ChIP Grade images

    • ab5338 staining Pdd1 in wild-type Tetrahymena sp. cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in methanol. The primary antibody was diluted 1/250 in TBS + 1% Goat Serum and incubated with the sample for 18 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.

      See Abreview

    • All lanes : Anti-Pdd1 antibody - ChIP Grade (ab5338) at 1/1000 dilution

      Lane 1 : Wild-type Tetrahymena sp. Starved Tetrahymena, do not express pdd1p (hour 0)
      Lane 2 : Wild-type Tetrahymena sp. Conjugating Tetrahymena, peak of pdd1p expression (hour 9)

      Lysates/proteins at 15000 cells per lane.

      Secondary
      HRP-conjugated Goat anti-Rabbit at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 64 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      This image is courtesy of an anonymous Abreview.

      See Abreview

    References for Anti-Pdd1 antibody - ChIP Grade (ab5338)

    This product has been referenced in:
    • Schwope RM & Chalker DL Mutations in Pdd1 reveal distinct requirements for its chromodomain and chromoshadow domain in directing histone methylation and heterochromatin elimination. Eukaryot Cell 13:190-201 (2014). WB ; Tetrahymena . Read more (PubMed: 24297443) »
    • Papazyan R  et al. Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis. Elife 3:e02996 (2014). WB ; Tetrahymena . Read more (PubMed: 25161194) »

    See all 10 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Tetrahymena sp. Cell lysate - whole cell (wildtype)
    Loading amount 15000 cells
    Specification wildtype
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 22 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Tetrahymena sp. Cell (wildtype)
    Specification wildtype
    Fixative Methanol
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"