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Rabbit polyclonal to PDE10A - C-terminal
Predicted to work with:
Recombinant fragment corresponding to a region within C terminal end amino acids 547 and 779 of PDE10A (Q9Y233).
Hela cells and whole cell lysate, Cal27 xenograft tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 88 kDa.
1/100 - 1/1000.
1/100 - 1/1000.
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate.
Abundant in the putamen and caudate nucleus regions of brain and testis, moderately expressed in the thyroid gland, pituitary gland, thalamus and cerebellum.
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
The tandem GAF domains bind cAMP, and regulate enzyme activity. The binding of cAMP stimulates enzyme activity.
Composed of a C-terminal catalytic domain containing two divalent metal sites and an N-terminal regulatory domain which contains one cyclic nucleotide-binding region.
Cytoplasm. Located mostly to soluble cellular fractions.
Information by UniProt
5''-cyclic phosphodiesterase 10A antibody
cAMP and cAMP inhibited cGMP 3'5' cyclic phosphodiesterase 10A antibody
cAMP and cAMP-inhibited cGMP 3'' antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE10A antibody - C-terminal (ab151454)
Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labeling PDE10A with ab151454 at 1/500.
Western blot - Anti-PDE10A antibody - C-terminal (ab151454)
Anti-PDE10A antibody - C-terminal (ab151454) at 1/3000 dilution + Hela whole cell lysate at 30 µg
Predicted band size: 88 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-PDE10A antibody - C-terminal (ab151454)
Immunofluorescence analysis of methanol fixed HeLa cells labeling PDE10A with ab151454 at 1/200. The lower panel shows costaining with Hoechst 33342.
has not yet been referenced specifically in any publications.
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