Overview

  • Product name
  • Description
    Goat polyclonal to PDE11A
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-NSFKESMEKSSYSD

    , corresponding to internal sequence amino acids 455-468 of Human PDE11A (NP_058649.3; NP_001070665.1; NP_001070826.1; NP_001070664.1)

  • Positive control
    • Human prostate tissue; Human prostate lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 105 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively.
  • Tissue specificity
    Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed in adrenal cortex.
  • Involvement in disease
    Defects in PDE11A are the cause of primary pigmented nodular adrenocortical disease type 2 (PPNAD2) [MIM:610475]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. PPNAD2 is characterized by adrenal glands with overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. PPNAD2 leads to Cushing syndrome.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Domain
    The tandem GAF domains bind cGMP, and regulate enzyme activity. The binding of cGMP leading to stimulate the enzyme activity.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic-AMP and -GMP phosphodiesterase 11A antibody
    • cAMP and cGMP phosphodiesterase 11A antibody
    • cGMP phosphodiesterase 11A antibody
    • Cyclic nucleotide phosphodiesterase 11A antibody
    • Dual 3'' antibody
    • Dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A antibody
    • pde11 antibody
    • PDE11_HUMAN antibody
    • pde11a antibody
    • PDE11A1 antibody
    • pde11a2 antibody
    • Phosphodiesterase 11A antibody
    • PPNAD2 antibody
    see all

Images

  • ab115624, at 10 µg/ml, staining PDE11A in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-PDE11A antibody (ab115624) at 1 µg/ml + Human prostate lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 105 kDa

References

ab115624 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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