Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE1A
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 212-520 of Human PDE1A (NP_001003683).

  • Positive control
    • EB: H1299 and HeLa whole cell lysates; 293T, A431, H1299, HepG2, MOLT4 and Raji cell lysates ICC/If: HeLa cell

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP.
  • Tissue specificity
    Several tissues, including brain, kidney, testes and heart.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3' 5' cyclic nucleotide phosphodiesterase antibody
    • 5''-cyclic nucleotide phosphodiesterase 1A antibody
    • 61 kDa Cam PDE antibody
    • 61 kDa Cam-PDE antibody
    • Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A antibody
    • Calcium/Calmodulin stimulated cyclic nucleotide phosphodiesterase antibody
    • Calcium/calmodulin-dependent 3'' antibody
    • Calmodulin-dependent Phosphodiesterase antibody
    • Cam PDE 1A antibody
    • Cam-PDE 1A antibody
    • CAM-PDE-1A antibody
    • HCAM 1 antibody
    • hCam-1 antibody
    • HCAM1 antibody
    • HSPDE1A antibody
    • MGC26303 antibody
    • PDE 1A antibody
    • PDE1A antibody
    • PDE1A_HUMAN antibody
    • Phosphodiesterase 1A antibody
    • Phosphodiesterase 1A calmodulin dependent antibody
    see all

Images

  • All lanes : Anti-PDE1A antibody (ab96336) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa
  • ab96336, at a 1/200 dilution, staining PDE1A in paraformaldehyde fixed HeLa by Immunofluorescence.
    Lower image is merged with DNA probe.

References

This product has been referenced in:
  • Li L  et al. Adenoviral short hairpin RNA therapy targeting phosphodiesterase 5a relieves cardiac remodeling and dysfunction following myocardial infarction. Am J Physiol Heart Circ Physiol 302:H2112-21 (2012). WB . Read more (PubMed: 22447941) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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