Recombinant
RabMAb

Anti-PDE1B antibody [EPR12293(2)] (ab182565)

Overview

  • Product name
    Anti-PDE1B antibody [EPR12293(2)]
    See all PDE1B primary antibodies
  • Description
    Rabbit monoclonal [EPR12293(2)] to PDE1B
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human C1orf101 aa 1-150. The exact sequence is proprietary.
    Database link: Q01064

  • Positive control
    • Human cerebellum and fetal brain tissue lysates; Human cerebellum tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12293(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P 1/100.

Target

  • Function
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic nucleotide phosphodiesterase 1B antibody
    • 63 kDa Cam PDE antibody
    • 63 kDa Cam-PDE antibody
    • Calcium/calmodulin dependent 3' 5' cyclic nucleotide Phosphodiesterase 1B antibody
    • Calcium/calmodulin stimulated cyclic nucleotide phosphodiesterase antibody
    • Calcium/calmodulin-dependent 3'' antibody
    • Calmodulin stimulated phosphodiesterase PDE1B1 antibody
    • Cam PDE 1B antibody
    • Cam PDE1B antibody
    • Cam-PDE 1B antibody
    • PDE 1B antibody
    • PDE1B antibody
    • PDE1B_HUMAN antibody
    • PDE1B1 antibody
    • PDES 1B antibody
    • PDES1B antibody
    • Phosphodiesterase 1B antibody
    • Phosphodiesterase 1B calmodulin dependent antibody
    • Presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling PDE1B with ab182565 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Anti-PDE1B antibody [EPR12293(2)] (ab182565) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • Anti-PDE1B antibody [EPR12293(2)] (ab182565) at 1/5000 dilution + Human cerebellum lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

References

ab182565 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Striatal Neuron)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Striatal Neuron
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Apr 13 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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