Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE3A
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, from the C-terminal region of Human PDE3A.

  • Positive control
    • Lysate from COS cells transfected with Human PDE isoforms.

Properties

Applications

Our Abpromise guarantee covers the use of ab125676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 125 kDa.

Target

  • Function
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase A antibody
    • cAMP phosphodiesterase myocardial cGMP inhibited antibody
    • CGI PDE antibody
    • CGI PDE A antibody
    • CGI-PDE A antibody
    • cGMP inhibited 3' 5' cyclic phosphodiesterase A antibody
    • cGMP-inhibited 3'' antibody
    • Cyclic GMP inhibited phosphodiesterase A antibody
    • Cyclic GMP-inhibited phosphodiesterase A antibody
    • Human cGMP inhibited cAMP phosphodiesterase mRNA antibody
    • PDE 3A antibody
    • Pde3a antibody
    • PDE3A_HUMAN antibody
    • Phosphodiesterase 3A antibody
    • Phosphodiesterase 3A cGMP inhibited antibody
    see all

Images

  • All lanes : Anti-PDE3A antibody (ab125676) at 1/2000 dilution

    Lane 1 : COS cell lysate, non-transfected
    Lane 2 : COS cell lysate, transfected with Human PDE1A3
    Lane 3 : COS cell lysate, transfected with Human PDE2A3
    Lane 4 : COS cell lysate, transfected with Human PDE3A
    Lane 5 : COS cell lysate, transfected with Human PDE3B
    Lane 6 : COS cell lysate, transfected with Human PDE4D2
    Lane 7 : COS cell lysate, transfected with Human PDE5A1
    Lane 8 : COS cell lysate, transfected with Human PDE5A3
    Lane 9 : COS cell lysate, transfected with Human PDE7A1
    Lane 10 : COS cell lysate, transfected with Human PDE8A1
    Lane 11 : COS cell lysate, transfected with Human PDE10A1
    Lane 12 : COS cell lysate, transfected with Human PDE11A1


    Predicted band size : 125 kDa

References

ab125676 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Heart Tissue)
Permeabilization
Yes - 1% Triton x-100
Specification
Heart Tissue
Blocking step
CasBlock as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up