Overview

  • Product name
  • Description
    Rabbit polyclonal to PDE3A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 450 and 500 (LPTLEPAPVR RDRSTSIKLQ EAPSSSPDSW NNPVMMTLTK SRSFTSSYAI S) of Human PDE3A (NP_000912.3).

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 125 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase A antibody
    • cAMP phosphodiesterase myocardial cGMP inhibited antibody
    • CGI PDE antibody
    • CGI PDE A antibody
    • CGI-PDE A antibody
    • cGMP inhibited 3' 5' cyclic phosphodiesterase A antibody
    • cGMP-inhibited 3'' antibody
    • Cyclic GMP inhibited phosphodiesterase A antibody
    • Cyclic GMP-inhibited phosphodiesterase A antibody
    • Human cGMP inhibited cAMP phosphodiesterase mRNA antibody
    • PDE 3A antibody
    • Pde3a antibody
    • PDE3A_HUMAN antibody
    • Phosphodiesterase 3A antibody
    • Phosphodiesterase 3A cGMP inhibited antibody
    see all

Images

  • All lanes : Anti-PDE3A antibody (ab99236) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 125 kDa


    Exposure time : 3 minutes
  • Detection of PDE3A in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab99236 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.

References

ab99236 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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