Recombinant
RabMAb

Anti-PDE3A antibody [EPR11601] (ab169534)

Overview

  • Product name
    Anti-PDE3A antibody [EPR11601]
    See all PDE3A primary antibodies
  • Description
    Rabbit monoclonal [EPR11601] to PDE3A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human PDE3A (UniProt Q14432).

  • Positive control
    • Fetal heart, HeLa and MCF7 lysates; Immunoprecipitation pellet from fetal heart lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 125 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
    • Sequence similarities
      Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5''-cyclic phosphodiesterase A antibody
      • cAMP phosphodiesterase myocardial cGMP inhibited antibody
      • CGI PDE antibody
      • CGI PDE A antibody
      • CGI-PDE A antibody
      • cGMP inhibited 3' 5' cyclic phosphodiesterase A antibody
      • cGMP-inhibited 3'' antibody
      • Cyclic GMP inhibited phosphodiesterase A antibody
      • Cyclic GMP-inhibited phosphodiesterase A antibody
      • Human cGMP inhibited cAMP phosphodiesterase mRNA antibody
      • PDE 3A antibody
      • Pde3a antibody
      • PDE3A_HUMAN antibody
      • Phosphodiesterase 3A antibody
      • Phosphodiesterase 3A cGMP inhibited antibody
      see all

    Images

    • All lanes : Anti-PDE3A antibody [EPR11601] (ab169534) at 0.43 µg/ml

      Lane 1 : Mouse brain tissue lysates
      Lane 2 : Mouse heart tissue lysates
      Lane 3 : Mouse kidney tissue lysates
      Lane 4 : Mouse spleen tissue lysates
      Lane 5 : Rat brain tissue lysates
      Lane 6 : Rat heart tissue lysates
      Lane 7 : Rat kidney tissue lysates
      Lane 8 : Rat spleen tissue lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 125 kDa
      Observed band size: 110 kDa (why is the actual band size different from the predicted?)


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-PDE3A antibody [EPR11601] (ab169534) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : HeLa lysate
      Lane 3 : MCF7 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 125 kDa

    • Anti-PDE3A antibody [EPR11601] (ab169534) at 1/1000 dilution + Immunoprecipitation pellet from Human fetal heart lysate

      Predicted band size: 125 kDa

    References

    ab169534 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (Heart)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein A
    Specification
    Heart
    Username

    Mrs. Andrea Geelhaar

    Verified customer

    Submitted Aug 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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