Anti-PDE4A antibody [6B6] (ab123925)


  • Product nameAnti-PDE4A antibody [6B6]
    See all PDE4A primary antibodies
  • Description
    Mouse monoclonal [6B6] to PDE4A
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human PDE4A produced in HEK293T cells (NP_006193).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY PDE4A cDNA; Human colon, liver and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY PDE4A.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.17% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab123925 is purified from Mouse ascites fluid by affinity chromatography.
  • ClonalityMonoclonal
  • Clone number6B6
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab123925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/4000. Predicted molecular weight: 98 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.


  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificityIsoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • Cellular localizationCytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 4A antibody
    • cAMP specific phosphodiesterase antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
    • DPDE2 antibody
    • dunce like phosphodiesterase E2 antibody
    • PDE 4A antibody
    • PDE4 antibody
    • PDE46 antibody
    • PDE4A antibody
    • PDE4A_HUMAN antibody
    • PDE4A11 antibody
    • Phosphodiesterase 4A antibody
    • phosphodiesterase 4A cAMP specific antibody
    • Phosphodiesterase 4A, cAMP-specific (dunce antibody
    • phosphodiesterase E2 dunce homolog antibody
    • Phosphodiesterase E2 dunce homolog, Drosophila antibody
    • phosphodiesterase isozyme 4 antibody
    • phosphodiesterase type 4A11 antibody
    see all

Anti-PDE4A antibody [6B6] images

  • ab123925 at 1/100 dilution staining PDE4A in HEK293T cells transfected with either pCMV6-ENTRY PDE4A (Red) or empty vector control plasmid (Blue) by flow cytometry.
  • ab123925 at 1/100 dilution staining PDE4A in COS7 cells transiently transfected with pCMV6-ENTRY PDE4A by Immunofluorescence.
  • ab123925 at 1/50 dilution staining PDE4A in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
  • ab123925 at 1/50 dilution staining PDE4A in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • ab123925 at 1/50 dilution staining PDE4A in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • All lanes : Anti-PDE4A antibody [6B6] (ab123925) at 1/4000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PDE4A cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 98 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC207765)

References for Anti-PDE4A antibody [6B6] (ab123925)

ab123925 has not yet been referenced specifically in any publications.

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I have been in contact with the lab about this product, the epitope that this product recognizes is not mapped, so we don't know if it would recognize all the isoforms of PDE4A.
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