Overview

  • Product name
    Anti-PDE4A (phospho S) antibody
    See all PDE4A primary antibodies
  • Description
    Rabbit polyclonal to PDE4A (phospho S)
  • Specificity
    This antibody detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phophorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with PDE4B, PDE4D or any other member of the PDE family.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine.

  • General notes


    This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The phospho-specific antibodies were selected by differential affinity chromatography matrices of phospho and non-phospho peptide columns.
  • Primary antibody notes
    This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 71 kDa (predicted molecular weight: 66 kDa). Detects bands at 66 kDa (PDE4A1), 109 kDa (PDE4A5), 106 kDa(PDE4A8), 102 kDa (PDE4Ax) and 76 kDa (testis specific PDE4A variant).
IP Use at an assay dependent dilution.

Target

  • Function
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificity
    Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level).
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications
    Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA.
    Proteolytically cleaved by caspase-3.
  • Cellular localization
    Cytoplasm > perinuclear region; Cytoplasm > perinuclear region. Cell projection > ruffle membrane; Cytoplasm. Membrane. Predominantly cytoplasmic and Membrane. Isoform 4 has propensity for association with membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 4A antibody
    • cAMP specific phosphodiesterase antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4A antibody
    • DPDE2 antibody
    • dunce like phosphodiesterase E2 antibody
    • PDE 4A antibody
    • PDE4 antibody
    • PDE46 antibody
    • PDE4A antibody
    • PDE4A_HUMAN antibody
    • PDE4A11 antibody
    • Phosphodiesterase 4A antibody
    • phosphodiesterase 4A cAMP specific antibody
    • Phosphodiesterase 4A, cAMP-specific (dunce antibody
    • phosphodiesterase E2 dunce homolog antibody
    • Phosphodiesterase E2 dunce homolog, Drosophila antibody
    • phosphodiesterase isozyme 4 antibody
    • phosphodiesterase type 4A11 antibody
    see all

Anti-PDE4A (phospho S) antibody images

  • Anti-PDE4A (phospho S) antibody (ab14610) at 1 µg/ml + Human thyroid normal tissue lysate - total protein (ab29636) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-PDE4A (phospho S) antibody (ab14610)

This product has been referenced in:
  • Schaal SM  et al. The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant following Spinal Cord Injury. PLoS One 7:e43634 (2012). WB ; Rat . Read more (PubMed: 23028463) »
  • Zhu B  et al. Activation and induction of cyclic AMP phosphodiesterase (PDE4) in rat pulmonary microvascular endothelial cells. Biochem Pharmacol 68:479-91 (2004). Read more (PubMed: 15242814) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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