Overview

  • Product nameAnti-PDE4B antibody
    See all PDE4B primary antibodies
  • Description
    Goat polyclonal to PDE4B
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    C-DIDIATEDKSPVDT

    , corresponding to C terminal amino acids 723-736 of Human PDE4B.

  • Positive control
    • Human prostate tissue; Mouse brain lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 83 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
  • Tissue specificityExpressed in brain, heart, lung and skeletal muscle.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 4B antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
    • cAMP-specific phosphodiesterase-4 B isoform antibody
    • DKFZp686F2182 antibody
    • DPDE4 antibody
    • dunce-like phosphodiesterase E4 antibody
    • MGC126529 antibody
    • OTTHUMP00000010652 antibody
    • OTTHUMP00000010653 antibody
    • OTTHUMP00000010654 antibody
    • OTTHUMP00000010656 antibody
    • OTTHUMP00000232365 antibody
    • PDE 4B antibody
    • PDE IVB antibody
    • PDE32 antibody
    • Pde4b antibody
    • PDE4B_HUMAN antibody
    • PDE4B5 antibody
    • PDEIVB antibody
    • Phosphodiesterase 4B antibody
    • Phosphodiesterase 4B cAMP specific antibody
    • Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) antibody
    see all

Anti-PDE4B antibody images

  • ab112014, at 5µg/ml, staining PDE4B in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • Anti-PDE4B antibody (ab112014) at 0.5 µg/ml + Mouse Brain lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 83 kDa

References for Anti-PDE4B antibody (ab112014)

ab112014 has not yet been referenced specifically in any publications.

Product Wall


Have you considered using a postive control: like a mouse brain lysate (ab7188)or prostate tissue (ab30304) to see if it works. This antibody has been tested on these tissue and is known to work. Since you are unsure of the expression in your wt s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"