Overview

  • Product name
  • Description
    Goat polyclonal to PDE4B
  • Specificity
    This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide

    DIDIATEDKSPVDT

    , from the C-terminal region of Human PDE4B

  • Positive control
    • Mouse brain lysate. Lysate prepared from transfected HEK293 cells transiently expressing Human PDE4B.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 83 kDa).

Target

  • Function
    Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
  • Tissue specificity
    Expressed in brain, heart, lung and skeletal muscle.
  • Pathway
    Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Sequence similarities
    Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5''-cyclic phosphodiesterase 4B antibody
    • cAMP-specific 3'' antibody
    • cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody
    • cAMP-specific phosphodiesterase-4 B isoform antibody
    • DKFZp686F2182 antibody
    • DPDE4 antibody
    • dunce-like phosphodiesterase E4 antibody
    • MGC126529 antibody
    • OTTHUMP00000010652 antibody
    • OTTHUMP00000010653 antibody
    • OTTHUMP00000010654 antibody
    • OTTHUMP00000010656 antibody
    • OTTHUMP00000232365 antibody
    • PDE 4B antibody
    • PDE IVB antibody
    • PDE32 antibody
    • Pde4b antibody
    • PDE4B_HUMAN antibody
    • PDE4B5 antibody
    • PDEIVB antibody
    • Phosphodiesterase 4B antibody
    • Phosphodiesterase 4B cAMP specific antibody
    • Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila) antibody
    see all

Images

  • Anti-PDE4B antibody (ab77272) at 0.5 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • Anti-PDE4B antibody (ab77272) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing Human PDE4B

    Predicted band size : 83 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

References

ab77272 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab77272.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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