Overview

  • Product nameAnti-PDE4D antibody
    See all PDE4D primary antibodies
  • Description
    Goat polyclonal to PDE4D
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    QPEACVIDDRSPDT

    , corresponding to C terminal amino acids 796-809 of Human PDE4D.

  • Positive control
    • Human Heart and Human Skeletal Muscle tissue; COS cell lysates transfected with Human PDE4D1 and transfected with Human PDE4D3

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab112018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 91 kDa.
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/128000.

Target

  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificityWidespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in diseaseNote=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications
    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localizationCytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D antibody images

  • Immunohistochemical analysis of PDE4D expression in formalin-fixed, paraffin-embedded Human heart tissue using ab112018 at 5 µg/ml.
  • Immunohistochemical analysis of PDE4D expression in formalin-fixed, paraffin-embedded Human skeletal muscle tissue using ab112018 at 5 µg/ml.
  • All lanes : Anti-PDE4D antibody (ab112018) at 1 µg/ml

    Lane 1 : COS cell lysates transfected with Human PDE4D1
    Lane 2 : COS cell lysates transfected with Human PDE4D3
    Lane 3 : COS cell lysates untransfected

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 91 kDa

References for Anti-PDE4D antibody (ab112018)

ab112018 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"