Anti-PDE4D antibody (ab14613)
Key features and details
- Rabbit polyclonal to PDE4D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE4D antibody
See all PDE4D primary antibodies -
Description
Rabbit polyclonal to PDE4D -
Host species
Rabbit -
Specificity
ab14613 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9 or PDE4C. The antibody has not been tested against endogenous PDE4D, only recombinant proteins and we therefore cannot guarantee it will detect endogenous PDE4. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to PDE4D. C-terminal cyclic peptide unique to all PDE4D subtypes variants.
(Peptide available asab169991) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500.
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IHC-P |
Use at an assay dependent concentration.
|
Notes |
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WB
1/500. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
Tissue specificity
Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Involvement in disease
Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
Post-translational
modificationsIsoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. -
Cellular localization
Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 5144 Human
- Omim: 600129 Human
- SwissProt: Q08499 Human
- Unigene: 117545 Human
- Unigene: 721404 Human
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Alternative names
- cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
- DKFZp686M11213 antibody
- 5''-cyclic phosphodiesterase 4D antibody
see all
Images
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Western blot using ab14613 and positive controls for PDE4D variants.
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ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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Datasheet download
References (18)
ab14613 has been referenced in 18 publications.
- Huang M et al. Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D. Mol Metab 47:101182 (2021). PubMed: 33549845
- Eyster CA et al. Diabetes induced decreases in PKA signaling in cardiomyocytes: The role of insulin. PLoS One 15:e0231806 (2020). PubMed: 32817622
- Zhou T et al. LncRNA XIST regulates myocardial infarction by targeting miR-130a-3p. J Cell Physiol 234:8659-8667 (2019). PubMed: 29226319
- Civciristov S et al. Preassembled GPCR signaling complexes mediate distinct cellular responses to ultralow ligand concentrations. Sci Signal 11:N/A (2018). PubMed: 30301787
- Pinto CS et al. Phosphodiesterase Isoform Regulation of Cell Proliferation and Fluid Secretion in Autosomal Dominant Polycystic Kidney Disease. J Am Soc Nephrol 27:1124-34 (2016). PubMed: 26289612