Overview

  • Product nameAnti-PDE4D antibody
    See all PDE4D primary antibodies
  • Description
    Rabbit polyclonal to PDE4D
  • Specificityab14613 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9 or PDE4C. The antibody has not been tested against endogenous PDE4D, only recombinant proteins and we therefore cannot guarantee it will detect endogenous PDE4.
  • Tested applicationsSuitable for: WB, IP, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    C-terminal cyclic peptide unique to all PDE4D subtypes variants.

  • Positive control
    • recombinant proteins (PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab14613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Can be blocked with PDE4D peptide (ab169991).
IP 1/150.
ICC 1/150.
ICC/IF Use at an assay dependent concentration. PubMed: 19759179
IHC-P Use at an assay dependent concentration.

Target

  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificityWidespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in diseaseNote=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications
    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localizationCytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D antibody images

  • ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab14613 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14613, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot using ab14613 and positive controls for PDE4D variants.

References for Anti-PDE4D antibody (ab14613)

This product has been referenced in:
  • He Z  et al. Ischemia-Induced Increase in Microvascular Phosphodiesterase 4D Expression in Rat Hippocampus Associated with Blood Brain Barrier Permeability: Effect of Age. ACS Chem Neurosci 3:428-32 (2012). IHC ; Rat . Read more (PubMed: 22860212) »
  • Wang X  et al. Cyclic nucleotide signaling in polycystic kidney disease. Kidney Int 77:129-40 (2010). IHC-P ; Mouse, Rat . Read more (PubMed: 19924104) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 80 µg
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Sample Mouse Cell lysate - whole cell (NIH3T3)
Specification NIH3T3
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Xuecai Ge

Verified customer

Submitted Apr 03 2014

Thank you for your reply and for your understanding in this matter.

As requested, I am sending ab14628 as a free of charge replacement for you and you should have it on Tuesday morning. For your records the new invoice number is *.

...

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I have tried 3 times to contact the supplier to ask them to provide those images, but they are not replying to my emails. I do apologize for the inconvenience this is causing you and I am grateful for your patience in the matter.

I believe th...

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Thank you for your patience in this matter.

I have heard back from the supplier in regards to ab14613 and they have tried to go back and look for old data on PDE4D antibody. They could not find the blot on HEK cells. They do have blots on oth...

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Thank you for contacting Abcam earlier today.

I am sorry about the western blot issues you have been having with ab14613. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . ...

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Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q...

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I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued free of charge replacements for ab14611 and ab14613with the order number 1137676.

To check the status...

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Thank you for contacting Abcam.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, ...

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Thank you for contacting us and sorry for the delay in response. I now have confirmation of the specificity of the two antibodies in question. ab14613 has been raised against a C-terminal peptide sequence of PDE4D. This peptide, contrary to what wa...

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Thank you for contacting us. Indeed, the annotation on the Western blot on our datasheet has an error. I have double checked this with our original data now. The bands are D1, D2, D3, D5 and D4. PDED5 is migrating at a lower molecular weight - as y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"