Overview

  • Product nameAnti-PDE4D antibody
    See all PDE4D primary antibodies
  • Description
    Goat polyclonal to PDE4D
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    QPEACVIDDRSPDT

    , corresponding to C terminal amino acids 670-673 of Human PDE4D (NP_006194.2).

  • Positive control
    • A549 cell lysate. PDE4D transfected COS-7 lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab77080 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 91 kDa.

Target

  • FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
  • Tissue specificityWidespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
  • PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
  • Involvement in diseaseNote=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution.
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
  • Post-translational
    modifications
    Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
  • Cellular localizationCytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
    • DKFZp686M11213 antibody
    • 5''-cyclic phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase 4D antibody
    • cAMP specific phosphodiesterase PDE4D6 antibody
    • cAMP-specific 3'' antibody
    • DPDE3 antibody
    • Dunce like phosphodiesterase E3 antibody
    • FLJ97311 antibody
    • HSPDE4D antibody
    • PDE43 antibody
    • Pde4d antibody
    • PDE4D_HUMAN antibody
    • PDE4DN2 antibody
    • Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3) antibody
    • Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila) antibody
    • Phosphodiesterase 4D cAMP specific antibody
    • Phosphodiesterase 4D, cAMP specific (dunce) antibody
    • STRK1 antibody
    see all

Anti-PDE4D antibody images

  • All lanes : Anti-PDE4D antibody (ab77080) at 1 µg/ml

    Lane 1 : COS cell lysates transfected with Human PDE4D isoform 1
    Lane 2 : COS cell lysates transfected with Human PDE4D isoform 3
    Lane 3 : COS cell lysates, non-transfected

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 91 kDa
    Observed band size : 68,95 kDa (why is the actual band size different from the predicted?)
  • Anti-PDE4D antibody (ab77080) at 0.3 µg/ml + A549 lysate in RIPA buffer at 35 µg

    Predicted band size : 91 kDa
    Observed band size : 70,80,90 kDa (why is the actual band size different from the predicted?)
  • Anti-PDE4D antibody (ab77080) at 0.3 µg/ml + lysates prepared from transfected HEK293 cells, transiently expressing PDE4D

    Predicted band size : 91 kDa

References for Anti-PDE4D antibody (ab77080)

This product has been referenced in:
  • Bazhin AV  et al. Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells. Mol Immunol 47:678-84 (2010). ELISA . Read more (PubMed: 19939455) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I apologise for the delay, I wanted to check this case with my lab colleagues.
Regarding ab59249, the amino acid range of this immunogen is aaXXXX of human PDE4D with accession# Q08499 (http://www.uniprot.org/uniprot/Q...

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Thank you for contacting us and sorry for the delay in response. I now have confirmation of the specificity of the two antibodies in question. ab14613 has been raised against a C-terminal peptide sequence of PDE4D. This peptide, contrary to what wa...

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